This web page was produced as an assignment for Genetics 564, an undergraduate capstone course at UW-Madison.
What is proteomics?
Proteomics is the study of the entire proteome, or set of proteins, associated with a cell or organism [1]. Not only are there thousands, upon thousands of distinctly different proteins within the human genome, there are even more proteins that arise due to the effect of post-translational modifications (Figure 1). Post-translational modifications include things like phosphorylation, glycosylation, methylation, and acetylation, and can affect the function of a particular protein with a cell [2].
The human proteome is dynamic, and it is ever-changing based upon various stimuli. Understanding how the proteome changes in response to diseases like myotonic dystrophy type 2 (DM2) offers insight into what proteins may be dysregulated proteins and what cellular pathways may be implicated in DM2.
What are the proteomic features of CNBP?
The first isoform is of the CNBP protein is one of six CNBP isoforms, and it is a 179 amino acids long [3]. Using NetPhos, it was found that the protein has a moderate potential for phosphorylation, with serine residues being more prevalent than both threonine and tyrosine residues (Figure 2). In addition, the protein has a theoretical pI/Mw of 7.76/19,690.95 according to the ExPASy database (Figure 3).
Discussion
Proteomics is the study of the collection of different protein forms found within a cell or organism. The proteome is dynamic, and changes can occur in diseased states due to various stimuli. CNBP is the primary gene that is mutated in DM2, thus leading to decreased expression of CNBP. Though expression of CNBP is decreased in DM2, some translation does still occur from the functioning gene copy and it may be important to study the effects that lower translation has on the post-translational modifications of CNBP. CNBP is also a protein that seems to regulate the functions of other genes, and through the use of PPIs, these target proteins can be identified and their proteomic changes in the disease state can be monitored. Continuing to research the post-translational modifications of CNBP itself and its interacting proteins may help offer deeper insight into the molecular basis of DM2.
References
- What is proteomics? (2016, June 08). Retrieved from https://www.ebi.ac.uk/training/online/course/proteomics-introduction-ebi-resources/what-proteomics
- Overview of Post-Translational Modifications (PTMs). (n.d.). Retrieved from https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-post-translational-modification.html
- CNBP CCHC-type zinc finger nucleic acid binding protein [Homo sapiens (human)] - Gene - NCBI. (n.d.). Retrieved from https://www.ncbi.nlm.nih.gov/gene/7555
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